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Application examples
Single cells in 30 minutes
Isolate single cells from suspensions such as buffy coat, cell cultures or directly from whole blood. No additional purification of the sample material is necessary. The bead catcher particles are directly mixed with the sample material and bind to the target cells. Employing a sieve-separation system the bound target cells are carefully separated from unbound material. Subsequently, the target cells are detached from the catcher particles under physiological conditions within 10 minutes. The target cells are now available as a single cells suspension and can be used for further experiments such as cell culture experiments, direct stimulation, or FACS analysis. For example, isolating CD3 cells from whole blood or buffy coat, you obtain single cells in 30 minutes.
RNA Isolation
RNA isolation of specific cells: You can isolate cells from complex biological solutions (whole blood or cord blood) for PCR analysis in less than 10 minutes.
Cell Activation
Activation of a cell sub-population: You can isolate your target cells and stimulate them with various concentrations of cell activators or for different activation durations. Cell stimulation can be performed right after the cell isolation. Because of the fast and simple cell isolation, several experiments can be performed simultaneously. As a quick rinse of cells is sufficient and centrifugation is not required at any step of the procedure even non-adherent cells can be activated in this way using MabTag‘s Beads&Sieve-technique.
Simultaneous Isolation
Simultaneous multi-target isolation. In case you handle only very small or particularly precious sample material, MabTag‘s Beads&Sieve-technique is the gold standard for your isolation experiment. Sample volumes of just 300 µl are sufficient for multiple target isolation experiments. Again, sample preparation is not necessary!
Depletion
Depletion: You can remove a particular population of cells from your sample. Just employ MabTag‘s Beads&Sieve-technique for a target cell or protein depletion (negative selection) and use the eluate for your experiment!
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